AATCC 174 pdf free download
AATCC 174 pdf free download.Antimicrobial Activity Assessment of Carpets.
4. Safety Precautions NOTE: These safely precautions are for information purposes only. The prc- cautions are ancillary to the testing proce- dures and are not intended to be all inclu- sive. It is the user’s responsibility to use safe and proper techniqucs in handling materials in this test method. Manufac- turers MUST be consulted for speeific details such as material safcty data sheets and other manufacturer ‘s recommenda- tions. All OSHA standards and rules must also be consulted and followed. 4.1 This test should be performed only by trained personnel. The U.S. Depar- ment of Health and Human Services pub- lication BiosufeT’ in Microbiological c1/ Biomedical Laborttories should be con- sulted (see 27.1). 4.2 CAUTION: Some of the microor- ganisms used in these tests are allergenic and pathogenic: i.e.. capable of infecting humans and producing discasc. There- fore. every necessary and reasonable pre- caution must be taken to climinate this risk to the laboratory pcrsonnel and to personnel in the associated environment. Wear protective clothing. respiratory pro- tection. and impervious gloves when working with the organismis. NOTE: Choose respiratory protcction that pre- vents penetration by the spores.4.3 Giood laboratory practices should be followed. Wear safely glasscs in all laboratory areas. 4.4 All chemicals should be handled with care. 4.5 An cycwash/safety shower should be located nearby for emergency use. 4.6 Sterilizc all contaminated samples and test materials prior to disposal. 4.7 Exposurc to chemicals used in this procedure must be controlled at or bclow levels set by government authorities (e.g.. Occupational Safcty and Health Admin- istration’s [OSHA | permissible exposure limits |PEL] as found in 29 CFR 1910. 1000 of January I. 1989). In addi- tion. the American Conference of Gov- ernmental Industrial Hygienists (ACGIHI) Threshold Limit Valucs (TLVs) com1- priscd of time weighted averagcs (TLV- T WA). short term exposure limits (TLV- STEL) and ceiling limits (TLV-C) are recommended as a general guide for air contaminant exposure which should be mct (sce 27.2).5. Uses and Limitations 5.1 This test method is designed for usC only for new carpcts and must not be used for carpets that have been laid down and worn. I. Qualitative Assessment of Antibacterial Activity on Carpets: Single Streak Method 6. Principle 6.1 Specimens of the test material, including corresponding untreated con- trols of the same material (if available but not required). are placed in intimate Con- tact with nutricnt agar which has bccn previously streaked with a bacterial cul- ture. Afier incubation. a clear area of interrupted growth undermneath and along the sides of the test material indicates antibacterial activity of the spccimen. Standard strains of bacteria are used with StuphloCOcCus cres (Gram positive) and Klebsiellu pentnonicne (Gram ncga- tive). the representative organisms. 7. Test Organisms 7.1 Staphwlococcus cures. American Type Culture Collection No. 6538 (scc 27.3).10. Test Specimens 10.1 Test specimens (non-sterile) are cut by hand or with a die. They may be any convenient size although oblong spee- imens cut 25 x 50 mm are recommended. 10.2 If possiblc. test a specimen of the same material treated in exactly the same way w ith whatever other finishing agents wcre used. but without the antibacterial agent. llowever, this is not cssential for the validity of the test. Mamy standard fin- ishing chemicals will give strong antibac- terial activity cven afier many clcanings. 11. Procedure 11.1 If durability data are desired. car- pet spccimens must be tested before and after being cleaned by a lest method agreed upon by the interested parties. 11.2 Dispense sterilized nutrient agar cooled to45土2°C (I13土4°F) by pour- ing 15.0 + 2.0 mL into each 100.0 mim di- ameter flat bottomed Petri dish. Alow agar to gel firmly before inoculating. 11.3 Prepare inoculum by transferring 1.0士0.1 mL ofa 24 h broth culture into 9.0土0.1 mL. of sterile distilled water contained in a test tube or small flask. Mix well using appropriate agitation.AATCC 174 pdf download.
