AATCC 147 pdf free download
AATCC 147 pdf free download.Antibacterial Activity Assessment of Textile Materials:Parallel Stfeak Method.
1. Purpose and Scope 1.1 The objective is to detect bacterio- static activity on textile materials. The re- sults of using this procedure have been demonstrated by Committee RA31 to be reproducible by various laboratories working with materials containing resid- ual amounts of antibacterial agents (as determined by chemical assay) after mul- tiple standard washings. The method is useful for obtaining a rough estimate of activity in that the growth of the inocu- lum organism decreases from one end of each streak to the other and from one streak to the next resulting in increasing degrees of sensitivity. The size of the zone of inhibition and the narrowing of the streaks caused by the presence of the antibacterial agent permit an estimate of the residual antibacterial activity after multiple washings.
3. Terminology 3.1 activity, n.- of an antibacterial agent, a measure of effctiveness of the agent. 3.2 antibacterial agent, n.- -in tex- tiles, any chemical which kills bacteria (bactericide) or interferes with the multi- plication, growth or activity of bacteria (bacteriostat). 3.3 zone of inhibition, n.- clear area of no growth of a microorganism, cul- tured onto the surface of an agar growth medium, in proximity to the borders of a specimen placed in direct contact with this agar surface. NOTE: A zone of inhibition occurs as a result of the diffusion of an antimicro- bial agent from the specimen.4. Safety Precautions NOTE: These safety precautions are for information purposes only. The pre- cautions are ancillary to the testing proce- dures and are not intended to be all inclu- sive. It is the user’s responsibility to use safe and proper techniques in handling materials in this test method. Manufac- turers MUST be consulted for specific details such as material safety data sheets and other manufacturer ‘s recommenda- tions. All OSHA standards and rules must also be consulted and followed. 4.1 This test should be performed only by trained personnel. The U.S. Depart- ment of Health and Human services pub- lication Biosafety in Microbiological and Biomedical Laboratories should be con- sulted (see 13.1).4.3 Good laboratory practices should be followed. Wear safety glasses in all laboratory areas. 4.4 All chemicals should be handled with care. 4.5 An eyewash/safety shower should be located nearby for emergency use. 4.6 Sterilize all contaminated samples and test materials prior to disposal. 4.7 Exposure to chemicals used in this procedure must be controlled at or below levels set by government authorities (e.g., Occupational Safety and Health Admin- istrations [OSHA] permissible exposure limits [PEL] as found in 29 CFR . 1910.1000 of January 1, 1989). In addi- tion, the American Conference of Gov- emmental Industrial Hygienists (ACGIH) Threshold Limit Values (TLVs) com- prised of time weighted averages (TLV- TWA), short term exposure limits (TLV- STEL) and ceiling limits (TLV-C) are recommended as a general guide for air contaminant exposure which should be met (see 13.2). 5. Uses and Limitations 5.1 The method is not suitable for ma- terials which tend to encapsulate and pre- vent the diffusion of the antibacterial agent or contain antibacterial-neutralizing substances.AATCC 147 pdf download.
