AATCC 147 pdf free download

AATCC 147 pdf free download.Test Method for Antibacterial Activity of Textile Materials: Parallel Streak.
2. Principle 2.1 Specimens of the test material, in- cluding corresponding untreated controls of the same material, are : placed in inti- mate contact with the agar surface which has been previously streaked with an in- oculum of a test bacterium. After incuba- tion, a clear area of interrupted growth underneath and along the sides of the test material indicates antibacterial activity of the specimen. A standard strain of bacte- ria is used which is specific to the re- quirements of the material under test. If no other bacterial species is specified, Staphylococcus aureus may be used as a representative Gram positive organism. Other recommended strains are listed be- low in Section 6. 3. Terminology 3.1 activity, n.- of an antibacterial agent, a measure of effectiveness of the agent. 3.2 antibacterial agent, n.- -any chemical which kills bacteria (bacteri- cide) or interferes with the multiplication, growth or activity of bacteria (bacteri- ostat). 3.3 zone of inhibition, n.- clear area of no growth of a microorganism, cul- tured onto the surface of an agar growth medium, in proximity to the borders of a specimen placed in direct contact with this agar surface.NOTE: A zone of inhibition occurs as a result of the diffusion of an antimicro- bial agent from the specimen. 4. Safety Precautions NOTE: These safety precautions are for information purposes only. The pre- cautions are ancillary to the testing proce- dures and are not intended to be all inclu- sive. It is the user ‘s responsibility to use safe and proper techniques in handling materials in this test method. Manufac- turers MUST be consulted for specific details such as material safety data sheets and other manufacturer ‘s recommenda- tions. All OSHA standards and rules must also be consulted and followed. 4.1 This test should be performed only by trained personnel. The U.S. Depart- ment of Health and Human services pub- lication Biosafety in Microbiological and Biomedical Laboratories should be con- sulted (see 12.1). 4.2 CAUTION: Some of the bacteria used in this test are pathogenic; i.e, capa- ble of infecting humans and producing disease. Therefore, every necessary and reasonable precaution must be taken to eliminate this risk to the laboratory per- sonnel and to personnel in the associated environment. Wear protective clothing and respiratory protection that prevents penetration by the bacteria. 4.3 Good laboratory practices should be followed. Wear safety glasses in all laboratory areas. 4.4 All chemicals should be handled with care. 4.5 An eyewash/safety shower should be located nearby for emergency use. 4.6 Sterilize all contaminated samples and test materials prior to disposal. 4.7 Exposure to chemicals used in this procedure must be controlled at or below levels set by government authorities (e.g., Occupational Safety and Health Administrations [OSHA] permissible ex- posure limits [PEL] as found in 29 CFR 1910.1000; see web site: www.osha.gov for latest version). In addition, the Ameri- can Conference of Governmental Indus- trial Hygienists (ACGIH) Threshold Limit Values (TLVs) comprised of time weighted averages (TLV-TWA)， short term exposure limits (TLV-STEL) and ceiling limits (TLV-C) are recommended as a general guide for air contaminant ex- posure which should be met (see 12.2).AATCC 147 pdf download.